Review



rabbit anti p eif2 α pabs  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc rabbit anti p eif2 α pabs
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    Rabbit Anti P Eif2 α Pabs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/Phospho-eIF2alpha+(Ser51)+XP+Rabbit+mAb/pmc13040927-66-76-83
    Average 96 stars, based on 1405 article reviews
    rabbit anti p eif2 α pabs - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2 α pathway"

    Article Title: The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2 α pathway

    Journal: Veterinary Research

    doi: 10.1186/s13567-025-01688-5

    pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    Figure Legend Snippet: pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.

    Techniques Used: Phospho-proteomics, Transfection, Western Blot, Incubation, Inhibition

    Related Articles

    other:

    Article Title: Mechanism of endoplasmic reticulum stress-induced vascular endothelial dysfunction
    Article Snippet: 53 Western blot analysis for P-eNOS, T-eNOS, P-PERK, T-PERK, BiP(GRP78), P-eIF2-α, T-eIF2-α, CHOP, P-p38 and T-p38MAPK, P-ERK1/2 and T-ERK1/2 (1:1000 dilution, Cell Signaling Technology, Inc, USA), and ATF6, HuR or β-actin (1:500 dilution, Santa Cruz Biotechnology, Inc) was performed using specific antibodies as previously described.

    Article Title: The envelope proteins from SARS-CoV-2 and SARS-CoV potently reduce the infectivity of human immunodeficiency virus type 1 (HIV-1)
    Article Snippet: The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2-α or total eIF2-α (both antibodies were obtained from Cell Signaling).

    Article Title: The Envelope Proteins from SARS-CoV-2 and SARS-CoV Potently Reduce the Infectivity of Human Immunodeficiency Virus type 1 (HIV-1)
    Article Snippet: The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2-α or total eIF2-α (both antibodies were obtained from Cell Signaling).



    Similar Products

    rabbit anti p eif2 α pabs  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rabbit anti p eif2 α pabs
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    Rabbit Anti P Eif2 α Pabs, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/Phospho-eIF2alpha+(Ser51)+XP+Rabbit+mAb/pmc13040927-66-76-83
    Average 96 stars, based on 1 article reviews
    rabbit anti p eif2 α pabs - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti p eif2 α  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti p eif2 α
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    Anti P Eif2 α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/Phospho-eIF2alpha+(Ser51)+Antibody/pm41588674-324-18-20
    Average 96 stars, based on 1 article reviews
    anti p eif2 α - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    5324t anti rabbit p eif2 α  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc 5324t anti rabbit p eif2 α
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    5324t Anti Rabbit P Eif2 α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/pm41566951-26-20-17
    Average 86 stars, based on 1 article reviews
    5324t anti rabbit p eif2 α - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    p eif2 α  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology p eif2 α
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    P Eif2 α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/eIF2%CE%B1+Antibody/pm40803544-81-7-27
    Average 96 stars, based on 1 article reviews
    p eif2 α - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    p eif2 α  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc p eif2 α
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    P Eif2 α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/pm40803544-81-7-19
    Average 86 stars, based on 1 article reviews
    p eif2 α - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    anti-p-eif2 α  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc anti-p-eif2 α
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    Anti P Eif2 α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/anti+4e+bp1/pmc12121226-86-6-9
    Average 90 stars, based on 1 article reviews
    anti-p-eif2 α - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    antibody p-eif2-α #3398  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc antibody p-eif2-α #3398
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    Antibody P Eif2 α #3398, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/anti+4e+bp1/pm40037075-67-17-30
    Average 90 stars, based on 1 article reviews
    antibody p-eif2-α #3398 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    p-eif2 α antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc p-eif2 α antibody
    pCP312R promotes the phosphorylation of PERK and <t>eIF2</t> α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis <t>using</t> <t>anti-Flag,</t> <t>anti-p-eIF2</t> α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
    P Eif2 α Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/anti+4e+bp1/pm39999483-62-6-20
    Average 90 stars, based on 1 article reviews
    p-eif2 α antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    p eif2 α s52  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc p eif2 α s52
    Reagents and tools table
    P Eif2 α S52, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-eif2-%CE%B1/Phospho-eIF2alpha+(Ser51)+XP+Rabbit+mAb/pmc11832788-41-0-4
    Average 96 stars, based on 1 article reviews
    p eif2 α s52 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.

    Journal: Veterinary Research

    Article Title: The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2 α pathway

    doi: 10.1186/s13567-025-01688-5

    Figure Lengend Snippet: pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.

    Article Snippet: Mouse anti-p72 monoclonal antibody (MAb) (catalog no. Anti PPA 1BC11, INGENASA), mouse anti-puromycin MAb (catalog no. MABE343, Sigma-Aldrich), rabbit anti- β -actin PAbs (catalog no. AC026, ABclonal), mouse anti-Flag MAb (catalog no. M8823, Sigma-Aldrich), mouse anti-GST MAb (catalog no. K200006M, Solarbio), rabbit anti-Myc PAbs (catalog no. YN5506, ImmunoWay), rabbit anti-KDEL PAbs (catalog no. ab214714, Abcam), rabbit anti-BiP PAbs (catalog no. A0241, ABclonal), mouse anti-PERK MAb (catalog no. 3192S, CST), rabbit anti-p-PERK PAbs (catalog no. BS66100, Bioworld), rabbit anti-p-eIF2 α PAbs (catalog no. 3398S, CST), and rabbit anti-eIF2 α PAbs (catalog no. 9722S, CST) are commercially available.

    Techniques: Phospho-proteomics, Transfection, Western Blot, Incubation, Inhibition

    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: The integrated stress response drives MET oncogene overexpression in cancers

    doi: 10.1038/s44318-024-00338-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: P-eIF2 α -S52 , Cell Signalling Technology , 3398.

    Techniques: Recombinant, Sequencing, Transfection, Western Blot, Luciferase, CyQUANT Assay, Proliferation Assay, Plasmid Preparation, Software